polyclonal goat igg primary antibody  (R&D Systems)

Journal: Heliyon

Article Title: The role of PDIA3 in oral squamous cell carcinoma and its value as A diagnostic and prognostic biomarker

doi: 10.1016/j.heliyon.2023.e22596

Figure Lengend Snippet: PDIA3 expression is up-regulated in OSCC tissues. (A and B) The mRNA expression of PDIA3 in the normal and OSCC tissues in the OSCC dataset from TCGA. (A) Compare all tumor and normal tissues. (B) Only compare paired tumors and normal tissues. (C) The protein expression of PDIA3 by IHC was retrieved from the HPA database. (D) The immunofluorescent staining of PDIA3 in human OSCC tissues and non-tumorous tissues. Left, representative images; right, quantification of the intensity of the PDIA3 staining. Bar = 20 μm. (E) The IHC staining of PDIA3 in human OSCC tissues and normal tissues. Left, representative IHC images. Upper: bar = 200 μm; lower: bar = 50 μm. Right, quantification of the intensity of the PDIA3 staining. (F) PDIA3 mRNA expression in normal tissues and tumors at different T stages. (G) PDIA3 level in normal tissues and OSCC tumors at different N stages. ***, P < 0.001. A P < 0.05 is significantly different.

Article Snippet: After overnight incubation at 4 °C with rabbit polyclonal anti-PDIA3 primary antibody (1:300, Proteintech, USA), the slides were incubated with HRP-goat anti-rabbit IgG secondary antibody (Boster Biological Technology Co. Ltd, China) at 37 °C for 30 min and washed with PBS three times.

Techniques: Expressing, Staining, Immunohistochemistry

Journal: Heliyon

Article Title: The role of PDIA3 in oral squamous cell carcinoma and its value as A diagnostic and prognostic biomarker

doi: 10.1016/j.heliyon.2023.e22596

Figure Lengend Snippet: The diagnostic and prognostic value of PDIA3 in OSCC. The PDIA3 expression and clinical data were retrieved from the OSCC dataset in TCGA. (A) The ROC curve for analyzing the value of PDIA3 in the diagnosis of OSCC. (B–D) The Kaplan-Meier method was used to analyze the value of PDIA3 in predicting the prognosis of OSCC patients. The Kaplan-Meier curves for (B) OS, (C) DSS and (D) PFI are shown here. The Logrank test was used to compare the curves. A P < 0.05 is significantly different.

Article Snippet: After overnight incubation at 4 °C with rabbit polyclonal anti-PDIA3 primary antibody (1:300, Proteintech, USA), the slides were incubated with HRP-goat anti-rabbit IgG secondary antibody (Boster Biological Technology Co. Ltd, China) at 37 °C for 30 min and washed with PBS three times.

Techniques: Diagnostic Assay, Expressing, Biomarker Discovery

Journal: Heliyon

Article Title: The role of PDIA3 in oral squamous cell carcinoma and its value as A diagnostic and prognostic biomarker

doi: 10.1016/j.heliyon.2023.e22596

Figure Lengend Snippet: Univariate and multivariate Cox regression analysis of PDIA3 in OSCC.

Article Snippet: After overnight incubation at 4 °C with rabbit polyclonal anti-PDIA3 primary antibody (1:300, Proteintech, USA), the slides were incubated with HRP-goat anti-rabbit IgG secondary antibody (Boster Biological Technology Co. Ltd, China) at 37 °C for 30 min and washed with PBS three times.

Techniques:

Journal: Heliyon

Article Title: The role of PDIA3 in oral squamous cell carcinoma and its value as A diagnostic and prognostic biomarker

doi: 10.1016/j.heliyon.2023.e22596

Figure Lengend Snippet: Down-regulation of PDIA3 inhibits the cancerous phenotypes of OSCC cells. The CAL27 and SCC25 cells were transfected with siRNA oligos targeting PDIA3 (designated as siPDIA3). Cells transfected with non-targeting oligos were used as controls (siNC). The untreated cells were also used and designated as ctrl. (A and B) Immunoblotting was performed to measure the protein level of PDIA3 in (A) CAL27 and (B) SCC25 cells. (C and D) The cell viability was measured by CCK8 assay on 24, 48, 72, and 96 h in (C) CAL27 and (D) SCC25 cells transfected with siPDIA3 or non-targeting siRNA. (E and F) The apoptosis of (E) CAL27 and (F) SCC25 cells was measured using an ELISA assay detecting the level of cytosolic nucleosomes. (G and H) The migratory ability of siRNA-transfected (G) CAL27 and (H) SCC25 cells was evaluated using a wound healing assay. Left: representative images; right: quantification of the wound healing rates. Scale bar = 100 μm *, P < 0.05, **, P < 0.01, ***, P < 0.001. A P < 0.05 is significantly different.

Article Snippet: After overnight incubation at 4 °C with rabbit polyclonal anti-PDIA3 primary antibody (1:300, Proteintech, USA), the slides were incubated with HRP-goat anti-rabbit IgG secondary antibody (Boster Biological Technology Co. Ltd, China) at 37 °C for 30 min and washed with PBS three times.

Techniques: Transfection, Western Blot, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Wound Healing Assay

Journal: Heliyon

Article Title: The role of PDIA3 in oral squamous cell carcinoma and its value as A diagnostic and prognostic biomarker

doi: 10.1016/j.heliyon.2023.e22596

Figure Lengend Snippet: PDIA3 regulates the PI3K/AKT signaling pathway in OSCC. (A) The mRNA expression of PI3KCD, AKT1-3, and mTOR in OSCC and normal tissues. (B) A heatmap showing the expression levels of PI3KCD, AKT1-3, and mTOR across PDIA3 high and PDIA3 low OSCC tissues. Red: high expression level; blue: low expression level. (C) The correlation between PDIA3 and PI3KCD, AKT1-3, or mTOR was analyzed by Spearman's correlation analysis. (D and E) PDIA3 was silenced by siRNA transfection (siPDIA3). Cells transfected with non-targeting siRNA (siNC) and untreated cells (ctrl) were used as controls. The levels of phosphor-AKT and total AKT were measured by immunoblotting in (D) CAL27 and (E) SCC25 cells. *, P < 0.05, **, P < 0.01, ***, P < 0.001. A P < 0.05 is significantly different.

Article Snippet: After overnight incubation at 4 °C with rabbit polyclonal anti-PDIA3 primary antibody (1:300, Proteintech, USA), the slides were incubated with HRP-goat anti-rabbit IgG secondary antibody (Boster Biological Technology Co. Ltd, China) at 37 °C for 30 min and washed with PBS three times.

Techniques: Expressing, Transfection, Western Blot

Journal: Heliyon

Article Title: The role of PDIA3 in oral squamous cell carcinoma and its value as A diagnostic and prognostic biomarker

doi: 10.1016/j.heliyon.2023.e22596

Figure Lengend Snippet: PDIA3 single-gene GSEA functional enrichment analysis. The GSEA for the differential genes related to PDIA3. (A) Enriched functions of the down-regulated genes. (B) Enriched functions of the up-regulated genes. (C) A lollipop plot showing the correlation between PDIA3 expression and immune cell infiltration in OSCC tissues by Spearman analysis. *, P < 0.05, **, P < 0.01, ***, P < 0.001, n.s, not significant. A P < 0.05 is significantly different.

Article Snippet: After overnight incubation at 4 °C with rabbit polyclonal anti-PDIA3 primary antibody (1:300, Proteintech, USA), the slides were incubated with HRP-goat anti-rabbit IgG secondary antibody (Boster Biological Technology Co. Ltd, China) at 37 °C for 30 min and washed with PBS three times.

Techniques: Functional Assay, Expressing

Journal: ACS biomaterials science & engineering

Article Title: Bioorthogonal "Click Chemistry" Bone Cement with Bioinspired Natural Mimicking Microstructures for Bone Repair.

doi: 10.1021/acsbiomaterials.2c01482

Figure Lengend Snippet: Figure 6. Angiogenesis. (a) Proliferation of HUVECs on the porous click-ON cements. (b) HUVEC morphology and distribution in the porous click cements with varied pore sizes. (c) mRNA expression levels of vascular markers in HUVECs growing on the cements. (d) Immunofluorescence staining of the vascular marker CD31 protein in HUVECs growing on the cements. (e) Schematic demonstration of potential support for vascular differentiation of stem cells on the porous click-ON cement and the mRNA expression level of vascular markers of (f) CD31, (g) α-SMA, (h) VEGFA, and (i) VEGFR2 in MSCs growing on the cements. (j) Immunofluorescence imaging of vascular marker CD31 protein expression in MSCs on the three types of click cements.

Article Snippet: To determine the local neovascularization in the rat cranial defect site, immunofluorescence co-staining was performed by incubating tissue sections with rabbit monoclonal anti-ALP primary antibodies (Novus Biologicals) and goat polyclonal anti-rat CD31 primary antibodies (Novus Biologicals).

Techniques: Expressing, Immunofluorescence, Staining, Marker, Imaging

Journal: ACS biomaterials science & engineering

Article Title: Bioorthogonal "Click Chemistry" Bone Cement with Bioinspired Natural Mimicking Microstructures for Bone Repair.

doi: 10.1021/acsbiomaterials.2c01482

Figure Lengend Snippet: Figure 8. In vivo osteogenesis and neovascularization. (a) In vivo osteogenic marker expression and (b) in vivo ALP activities in rat calvarial defect sites. Immunofluorescence intensity quantification of (c) ALP osteogenic maker and (d) CD31 vascular marker in tissue slices from the rat cranial defects with the PO-click-ON cement and the empty control. Immunohistochemistry (IHC)-stained images of ALP (green)/nuclei (blue) and CD31 (red)/nuclei (blue) in tissue slices from (e, f) the empty rat cranial defects and (g, h) the defects with the PO-click-ON cement. (i) Schematic demonstration of limited bone repair in the empty bone defect and the robust enhancement of bone repair by the PO-click-ON cement taking advantage of favorable cell recruitment, osteoinductivity, osteoconductivity, and neovascularization. Asterisk (*): statistically different (p < 0.05).

Article Snippet: To determine the local neovascularization in the rat cranial defect site, immunofluorescence co-staining was performed by incubating tissue sections with rabbit monoclonal anti-ALP primary antibodies (Novus Biologicals) and goat polyclonal anti-rat CD31 primary antibodies (Novus Biologicals).

Techniques: In Vivo, Marker, Expressing, Immunofluorescence, Control, Immunohistochemistry, Staining